Department of Molecular Biophysics

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Personal of the Department

Olga A. Ryazanova
 

Olga A. Ryazanova, Ph.D.
(born September 26, 1967)

Research fellow

Department of Molecular Bophysics
B.Verkin Institute for Low Temperature
Physics & Engineering, NAS of Ukraine

47 Lenin ave., Kharkov , 61103, Ukraine

Phone : (+380)-57-3410958

Fax : (+380)-57-3450593

e-mail: ryazanova@ilt.kharkov.ua

 

Education:

M.Sci – 1989  (physical optics and spectroscopy) , Dept. of physics , Kharkov State University, Kharkov , Ukraine

Ph.D –  2008  (thermal physics and molecular physics), B. Verkin Institute for Low Temperature Physics and Engineering NAS of Ukraine, Kharkov , Ukraine

Research areas: molecular spectroscopy, molecular biophysics, physical chemistry

Research Interests :

  • Effect of divalent metal ions on the conformational transitions in polynucleotide systems
  • Photophysical properties of intercalative dyes
  • Interaction of intercalative dyes with synthetic polynucleotides of various base compositions and secondary structures
  • Thermodynamics of the hybridization of antisense oligonucleotides and triplex forming agents modified by intercalating dyes
  • Self-assembly of porphyrin derivatives on polyionic biopolymer templates
  • Fluorescent probes for the recognition of self-assembled guanosine structures
  • Interaction of porphyrin derivatives with poly(G) and G-rich oligonucleotides forming G-quadruplexes
  • Targeting of G-quadruplex forming systems by small molecules to enhance their thermostability

Area of expertise:

conformational transitions in nucleic acids, effect of divalent metal ions, intercalative dyes, drug – nucleic acids interaction, thermodynamics of antisense oligonucletides and triplex forming agents modified by intercalative dyes, porphyrines, G-quadruplexes, self-assembly of porphyrins on polyionic matrices, UV-VIS absorption spectroscopy, polarized fluorescence spectroscopy, static light scattering, UV melting, fluorescent melting.

Technical skills:

  • UV-visible absorption spectroscopy
  • polarized fluorescent spectroscopy
  • static light scattering
  • melting technique with absorption and fluorescent registration of melting curves

Honours and Awards

1989 - Magister's diploma with first-class honour degree.
2004 – Winner (with co-authors) of Annual Prize of B.Verkin Institute for Low Temperature Physics & Engineering of NASU for best article in area of biophysics
2008 – Winner (with co-authors) of Annual Prize of B.Verkin Institute for Low Temperature Physics & Engineering of NASU for best article in area of biophysics

Participation in the International Joint Scientific Projects:

1999-2001 - grant No. 97-31158 of INTAS Program.
2002-2004 - grant UB2-2442-KH-02 of Civilian and Research Development Foundation (CRDF) Program.
2005-2006 - UNESCO/ IBSP Project 2-UA-03 (Contract No: 4500027456).
2005-2007 – grant No. 3172 of Science and Technology Center in Ukraine .

Languages: Russian and Ukrainian – native languages, fluent in English, understand Polish.

Resent Publications:

  1. V.N. Zozulya, O.A. Ryazanova, I.M. Voloshin, A.Yu.  Glamazda, V.A. Karachevtsev “ Spectroscopic Detection of Tetracationic Porphyrin H-Aggregation on Polyanionic Matrix of Inorganic Polyphosphate.” (in press).
  2. Î . À . Ryazanova, I. Ì . Voloshin, L.V. Dubey, V.N. Zozulya “Spectroscopic study of pheophorbide-a methyl ether binding to synthetic polynucleotides and DNA” Biophysical Bulletin (2009) v. 23 (2), p. 20-29 (in Russian).
    http://www-biomedphys.univer.kharkov.ua/JOURNAL/pdf/23_2.pdf
  3. O.A. Ryazanova, I.M. Voloshin, I.Ya. Dubey, L.V. Dubey , V.N. Zozulya " Fluorescent studies on cooperative binding of cationic Pheophorbide-a derivative to polyphosphate ", Annals of the New York Academy of Sciences (2008) v. 1130, p.  293-299. DOI: 10.1196/annals.1430.033
  4. O.A. Ryazanova, I.M. Voloshin, I.Ya. Dubey, L.V. Dubey and V.N. Zozuly a “Spectroscopic studies on binding of cationic Pheophorbide-a derivative to model polynucleotides” , IFMBE Proceedings (2008) v.20, p. 397-400. DOI: 10.1007/978-3-540-69367-3_107
  5. Î.À. Ryazanova, I.Ya. Dubey, V.N. Zozulya “Investigation of the effect of covalently attached phenazine dye on helix-to-coil transition in mixed poly(rA)-(dT) 14 system”, Biophysical Bulletin (2008) v.20 (1), p. 17-23. (in Russian) http://www-biomedphys.univer.kharkov.ua/JOURNAL/pdf/20_2.pdf
  6. O . A . Ryazanova , I . M . Voloshin , V . L . Makitruk , V . N . Zozulya , V . A . Karachevtsev " pH – Induced changes in electronic absorption and fluorescence spectra of phenazine derivatives ", Spectrochimica Acta Part A : Molecular and Biomolecular Spectroscopy (2007) v .   66 (4-5), p .   849-859. gggdoi :10.1016/ j . saa .2006.04.027
  7. Olga Ryazanova, Victor Karachevtsev, Igor Voloshin and Victor Zozulya. "Investigation of self-assembly of pheophorbide-a on poly-L-Lysine matrix", Journal of Porphyrins and Phthalocyanines (2006), v. 10 (4-6), p. 846.
  8. O.A. Ryazanova, V.N. Zozulya, I.M. Voloshin, V.A. Karachevtsev, V.L. Makitruk, S.G. Stepanian. “ Absorption and fluorescence spectral studies of imidazophenazine derivatives .” Spectrochimica Acta Part A (2004), v.60 (8-9), p. 2005-2011. doi:10.1016/j.saa.2003.10.020  
  9. Â.Í. Çîçóëÿ, À.Á. Íåñòåðîâ, Î.À. Ðÿçàíîâà, Þ.Ï. Áëàãîé "Âëèÿíèå èîíîâ Cd 2+ íà êîíôîðìàöèîííûå ïåðåõîäû â ñèñòåìå Poly ( dA )- Poly ( dT )". ³ñíèê Õàðê. Óí-òó (Á³îô³çè÷íèé ³ñíèê) 2004, ¹ 637, âèï. 1-2(14), ñ. 71-75.
  10. Zozulya V.N., Nesterov A.B., Ryazanova O.A., Blagoi Yu.P. “Conformational transitions and aggregation in poly(dA)-poly(dT) system induced by Na + and Mg 2+ ions.” The International Journal of Biological Macromolecules ( UK ) (2003) v. 33 (4-5), p. 183-191 . doi:10.1016/j.ijbiomac.2003.08.003
  11. V. Zozulya, Yu. Blagoi, I. Dubey, D. Fedoryak, V. Makitruk, O. Ryazanova, A. Shcherbakova “ Anchorage of an Oligonucleotide Hybridization by a Tethered Phenazine Nucleoside Analogue.” Biopolymers (Biospectroscopy) (2003) 72 (4), p. 264-273. Doi:10.1002/bip.10403
  12. Â.Í. Çîçóëÿ, Þ.Ï. Áëàãîé, Ä.Í. Ëîæêî, À.Á. Íåñòåðîâ, Î.À. Ðÿçàíîâà "Âëèÿíèå èîíîâ Ni 2+ íà êîíôîðìàöèîííûå ïåðåõîäû â ñèñòåìå Poly ( dA )- Poly ( dT ) " . ³ñíèê Õàðê. Óí-òó (Á³îô³çè÷íèé ³ñíèê) 2003, ¹ 606, âèï. 2(13), ñ. 43-47.
  13. Yu.P. Blagoi, V.N. Zozulya, S.A. Egupov, O.A. Ryazanova, A.S. Shcherbakova “Thermodynamic analysis of 3 ® 2 transition in oligonucleotide triple–helix complexes dA n -2dT m .”, Biophysical Bulletin (Visnyk Kharkivskogo universytetu, Ukraine ) ( 2002) v.  560, issue 1(10), p. 5-11.
  14.   Yu.P. Blagoi, V.N. Zozulya, S.A. Egupov, O.A. Ryazanova, A.S. Shcherbakova, “Thermodynamics of helix-coil transitions in duplex complexes of oligoadenilates with oligothymidilates.”, Biophysical Bulletin (Visnyk Kharkivskogo universytetu, Ukraine) ( 2001) v. 528, issue 2(9), p.10-16.